Simultaneous determination of calycosin, prim-O glucosylcimifugin, and paeoniflorin in rat plasma by HPLC-MS/MS: application in the pharmacokinetic analysis of HQCF
The Author(s) 2020
Article reuse guidelines: sagepub.com/journals-permissions
DOI: 10.1177/0300060520972902
journals.sagepub.com/home/imr
Yulong Gu1,2, Xianglan Piao1,2 and Dan Zhu
Abstract
Objective: This study aimed to develop and validate a high-performance liquid chromatography–
tandem mass spectrometry method to simultaneously determine three bioactive components of
the Huangqi Chifeng decoction (HQCF) in rat plasma.
Methods: Taxol was used as an internal standard in the developed method. Chromatographic
separation was performed on a C18 column using a gradient elution with 0.1% formic acid in
acetonitrile (v/v) and 0.1% formic acid in water (v/v) as the mobile phases at a flow rate of
0.4 mLminute1 . All compounds were monitored via selected reaction monitoring with an
electrospray ionization source.
CA, PG, PA, and Taxol were purchased from Chengdu DeSiTe Biological Technology Co. Ltd. (Chengdu, China).
The purities of all compounds were determined by HPLC (98%).
