A Rapid High Throughput Vibration and Vortex-Assisted Matrix Solid Phase Dispersion for Simultaneous Extraction of Four Isoflflavones for Quality Evaluation of Semen Sojae
Praeparatum
Xuejing Yang1,2† , Ali Sun1,3† , Evans Owusu Boadi 1,3 , Jin Li 1 *, Jun He1,3 , Xiu-mei Gao1 and
Yan-xu Chang1,3*
Isoflflavones (daidzein, daidzin, genistein and genistin) were main chemical
components and usually selected as markers for quality control of Traditional
Chinese Medicine semen sojae praeparatum (SSP). High throughput vibration
and vortex-assisted matrix solid phase dispersion and high performance liquid
chromatography with diode array detection were developed to simultaneously
extract and quantify four isoflflavones in SSP. Some parameters inflfluencing
extraction effificiency of isoflflavones by vortex-assisted matrix solid phase
dispersion such as sorbent type, ratio of sample to sorbent, crushing time,
vibration frequency, methanol concentration, eluting solvent volume and vortex
time were optimized. It was found that the best extraction yields of four
isoflflavones were obtained when the sample (20 mg) and SBA-3 (40 mg) was
crushed by ball mill machine for 2 min at vibration frequency of 800 times per
minute. Methanol/water (1.5 ml, 8:2, v/v) solution was dropped into the treated
sample and vortexed for 3 min. The recoveries of the four isoflflavones ranged from
86.1 to 94.8% and all relative standard deviations were less than 5%. A good linearity
(r > 0.9994) was achieved within the range 0.5–125 μg/ml. It was concluded that the
high throughput vibration and vortex-assisted matrix solid-phase dispersion
coupled with high performance liquid chromatography was user-friendly
extraction and quantifification method
Genistein, genistin, daidzein and daidzin (purity on HPLC > 98%) were supplied by Chengdu Desite Bio-Technology (Chengdu, China).
